小屋顶的嫩帅 小屋顶的嫩帅
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利用图书馆资源,查了点关于 红玫瑰 的东东。。有兴趣的来看看 ISI: 讲红玫瑰毒性的,主要是对Na+传导的影响 Isolation and characterization of a novel toxin from the venom of the spider Grammostola rosea that blocks sodium channels         作者: Clement H (Clement, Herlinda), Odell G (Odell, George), Zamudio FZ (Zamudio, Fernando Z.), Redaelli E (Redaelli, Elisa), Wanke E (Wanke, Enzo), Alagon A (Alagon, Alejandro), Possani LD (Possani, Lourival D.)   来源出版物: TOXICON     卷: 50     期: 1     页: 65-74     出版年: JUL 2007     被引频次: 2      参考文献: 33      引证关系图       摘要: This communication reports the chemical and physiological characterization of a novel peptide (GrTx1) isolated from the venom of the "rosean-tarantula" Grammostola rosea. This component was one among more than 15 distinct components separated from the soluble venom by high-performance liquid chromatography (HPLC). GrTx1 has 29 amino-acid residues, compactly folded by three disulfide bridges with a molecular weight of 3697 Da. Here we show that this peptide blocks Na+ currents of neuroblastoma F-11 cells with an IC50 of 2.8 +/- 0.1 mu M, up to a maximum of about 85% at 10 mu M. M oreover, the right-shift (+ 20.1 +/- 0.4 mV) of the fractional voltage-dependent cond uctance could be also compatible with a putative "gating-modifier" mechanism. No effects were seen on common K+ channels, such as K(v)1.1 and 1.4, using concentrations of toxin up to 10 mu M. Sequence analysis reveals that GrTx1 is closely related to other spider toxins reported to affect various distinct ion channel functions. A critical analysis of this study suggests the necessity to search for other potential receptor sites in order to establish the preferred specificity of these kind of peptides. (c) 2007 Elsevier Ltd. All rights reserved. 文献类型: Article   语言: English   作者关键词: Grammostola rosea; ion-channel; phylogenetic tree; primary structure; spider toxin; tarantula   KeyWords Plus: MECHANOSENSITIVE ION CHANNELS; GATED CALCIUM-CHANNELS; GATING MODIFIER TOXINS; OMEGA-GRAMMOTOXIN SIA; BETA-SCORPION TOXIN; POTASSIUM CHANNELS; PEPTIDE INHIBITOR; VOLTAGE; RELEASE; PHARMACOLOGY   通讯作者地址: Possani, LD (通讯作者), Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ,2001 Apartado Postal 510-3, Cuernavaca 62210, Morelos Mexico   地址: 1. Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62210, Morelos Mexico 2. Univ Milan, Dept Biotechnol & Biosci, I-20126 Biccoca, Italy   电子邮件地址: possani#ibt.unam.mx   出版商: PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND   IDS 号: 186DO   ISSN: 0041-0101   DOI: 10.1016/j.toxicon.2007.02.015  
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