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能帮忙翻译一下我的外文翻译吗?我翻译得不咋样! Potato Research (2006) 49:167-176DOT 10.1007/x11540-006-9015-0Application of Soluble Chitosan in vitro and in the Greenhouseto Increase Yield and Seed Quality of Potato MinitubersBritta Kowalsld•Felipe Jimenez Terry•Lidcay Herrera•Daniel Agramonte PeiialverReceived: 14 October 2005 /Accepted: 4 December 2006/Published online: 13 February 2007O') EAPR 2007Abstract The aim of this work was to investigate whether the application of soluble chitosanin potato micropropagation can improve microplant quality in vitro, help acclimatisation exvitro, and increase yield and seed quality of minitubers. Potato cv. Desiree microplants weretreated in vitro with soluble chitosan added to the semisolid tissue culture medium in differentconcentrations. Microplants were subsequently transferred to the greenhouse and sprayed withchitosan solutions or remained unsprayed. Untreated microplants were also established ex vitroand sprayed with chitosan, or left unsprayed as a control. Morphological and physiologicalparameters of plant growth were assessed in vitro and ex vitro. Plantlet quality was evaluatedusing a ranking system. Minitubers derived from greenhouse plantlets were planted in the fieldin subtropical conditions, and growth and yield parameters evaluated. The chitosanconcentration most beneficial to the in vitro growth of microplants varied between years. Intreatments with the best in vitro growth, minituber number and yield in the greenhouse was alsoincreased. Foliar chitosan application at the acclimatisation phase stabilised the effect of in vitrotreatment on yield parameters. The seed quality of minitubers derived from chitosan treatmentsin vitro alone and in combination with foliar treatment at acclimatisation was improved, givingrise to field plants with increased tuber numbers and yields. The combination of chitosan in vitroand foliar application during the acclimatisation phase ex vitro had a greater effect on minituberseed quality than the concentration applied in vitro.Keywords micropropagation•Solanum tubero.sum LB. Kowalski(皿)•F. J. Terry•L. Herrera•D. A. PenalverInstitute for Land Use, University of Rostock, 18051 Rostock, Germanye-mail: [email protected]. Kowalski•F. J. Terry•L. Herrera•D. A. PenalverInstitute of Plant Biotechnology, University of Santa Clara,Carretera Camajuani km 3.5, Villa Clara, Cuba】6RPotato Research (2006) 49:167-176lntrnductinnMicropropagation offers a method to produce healthy potato seed from disease freemicroplants (plantlets propagated in vitro) provided the cost intensive process can be madeefficient by producing high and stable yields and reliable seed quality to lower seed costs. Themain problem in commercial micropropagation are the stresses which tissue culture conditionsimpose on microplants through high osmoticity, abnormal mineral nutrition, unusualhormonal treatment, high relative humidity and accumulation of gasses such as ethylene,and the mechanical injury through dissection (Cassells and Walsh 1994; Gaspar et al. 2002;

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